Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 420082
Title Beta-carotene Reduces Body Adiposity of Mice Via BCMO1
Author(s) Amengual, J.; Gouranton, E.; Helden, Y.G.J.; Keijer, J.; Kramer, E.H.M.
Source PLoS One 6 (2011)6. - ISSN 1932-6203 - 13 p.
DOI https://doi.org/10.1371/journal.pone.0020644
Department(s) Human and Animal Physiology
Rikilt B&T Toxicologie en Effectanalyse
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) trans-retinoic acid - proliferator-activated receptors - vitamin-a production - diabetes-mellitus - gene-expression - metabolizing enzyme - leptin expression - serum carotenoids - nuclear receptors - ppar-gamma
Abstract Evidence from cell culture studies indicates that ß-carotene-(BC)-derived apocarotenoid signaling molecules can modulate the activities of nuclear receptors that regulate many aspects of adipocyte physiology. Two BC metabolizing enzymes, the BC-15,15'-oxygenase (Bcmo1) and the BC-9',10'-oxygenase (Bcdo2) are expressed in adipocytes. Bcmo1 catalyzes the conversion of BC into retinaldehyde and Bcdo2 into ß-10'-apocarotenal and ß-ionone. Here we analyzed the impact of BC on body adiposity of mice. To genetically dissect the roles of Bcmo1 and Bcdo2 in this process, we used wild-type and Bcmo1-/- mice for this study. In wild-type mice, BC was converted into retinoids. In contrast, Bcmo1-/- mice showed increased expression of Bcdo2 in adipocytes and ß-10'-apocarotenol accumulated as the major BC derivative. In wild-type mice, BC significantly reduced body adiposity (by 28%), leptinemia and adipocyte size. Genome wide microarray analysis of inguinal white adipose tissue revealed a generalized decrease of mRNA expression of peroxisome proliferator-activated receptor ¿ (PPAR¿) target genes. Consistently, the expression of this key transcription factor for lipogenesis was significantly reduced both on the mRNA and protein levels. Despite ß-10'-apocarotenoid production, this effect of BC was absent in Bcmo1-/- mice, demonstrating that it was dependent on the Bcmo1-mediated production of retinoids. Our study evidences an important role of BC for the control of body adiposity in mice and identifies Bcmo1 as critical molecular player for the regulation of PPAR¿ activity in adipocytes
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