Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 420089
Title Diversity in biofilm formation and production of curli fimbriae and cellulose of Salmonella Thyphimurium strains of different origin in high and low nutrient medium
Author(s) Castelijn, G.A.A.; Veen, S. van der; Zwietering, M.H.; Moezelaar, R.; Abee, T.
Source Biofouling 28 (2012)1. - ISSN 0892-7014 - p. 51 - 63.
DOI http://dx.doi.org/10.1080/08927014.2011.648927
Department(s) Food Microbiology Laboratory
FBR Food Technology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) enterica serovar typhimurium - air-liquid interface - listeria-monocytogenes - multicellular morphotype - gastrointestinal-tract - agfd promoter - behavior - enterobacteriaceae - enteritidis - persistence
Abstract The biofilm forming behavior of 51 Salmonella Typhimurium strains was determined in Tryptone Soya Broth (TSB) and 20 times diluted TSB (1/20TSB) at 25°C and 37°C. The results indicated that biofilm forming behavior is influenced by environmental conditions and associated with the origin of the strains. Clinical, outbreak-associated and retail product isolates showed dense biofilm formation in both media at 25°C, and in TSB also at 37°C. However, industrial isolates only showed dense biofilm formation in 1/20TSB at 25°C. By enumeration of biofilm cells, LIVE/DEAD staining and SEM analysis of biofilms it was found that the ratio of cells and extracellular matrix is affected by environmental conditions. Indeed, the genes involved in curli fimbriae and cellulose production are highly induced during biofilm formation at 25°C in 1/20TSB. This indicates that these are important matrix components during biofilm formation in 1/20TSB at 25°C and that other factors contribute to biofilm formation of clinical, outbreak-associated and retail product isolates at 37°C and/or nutrient-rich conditions
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