Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 420891
Title Opportunities and challenges for the baculovirus expression system
Author(s) Oers, M.M. van
Source Journal of Invertebrate Pathology 107 (2011)Suppl.. - ISSN 0022-2011 - p. S3 - S15.
DOI https://doi.org/10.1016/j.jip.2011.05.001
Department(s) Laboratory of Virology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) nuclear polyhedrosis-virus - envelope fusion protein - infected insect cells - swine-fever virus - occlusion-derived virus - n-linked glycosylation - escherichia-coli - endoplasmic-reticulum - surface display - recombinant baculoviruses
Abstract In this review background information on the baculovirus-insect cell expression system and its applications for producing protein subunits and virus-like particles for vaccine and other purposes is provided. This review will illustrate the principle structure of baculovirus vectors commonly used for heterologous gene expression in insect cells and describe adaptations that have been made over the last 10 years to improve the system in terms of quality of the protein produced and stability of the baculovirus genome. These improvements include enhanced trafficking, folding and glycosylation of the recombinant protein as well as preventing intracellular degradation. Challenges and progress in stabilizing the baculovirus genome in order not to lose the transgene cassette will also be discussed. Recent developments such as how to make multiple alterations in the baculovirus genome without accumulating marker genes are included
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