Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 421480
Title Surface charge-specific cytotoxicity and cellular uptake of tri-block copolymer nanoparticles
Author(s) Bhattacharjee, S.; Ershov, D.S.; Gucht, J. van der; Alink, G.M.; Rietjens, I.; Zuilhof, H.; Marcelis, A.T.M.
Source Nanotoxicology 7 (2013)1. - ISSN 1743-5390 - p. 71 - 84.
Department(s) Laboratory for Organic Chemistry
Physical Chemistry and Colloid Science
Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) supported lipid-bilayers - cerium oxide nanoparticles - gold nanoparticles - oxidative stress - gene-expression - in-vitro - manufactured nanoparticles - chitosan nanoparticles - silicon nanoparticles - mitochondrial damage
Abstract A series of monodisperse (45 ± 5 nm) fluorescent nanoparticles from tri-block copolymers (polymeric nanoparticles (PNPs)) bearing different surface charges were synthesised and investigated for cytotoxicity in NR8383 and Caco-2 cells. The positive PNPs were more cytotoxic and induced a higher intracellular reactive oxygen species production than the neutral and negative ones. The cytotoxicity of positive PNPs with quaternary ammonium groups decreased with increasing steric bulk. The intracellular uptake and cellular interactions of these different PNPs were also tested in NR8383 cells by confocal laser scanning microscopy, which revealed higher uptake for positive than for negative PNPs. Also positive PNPs were found to interact much more with cell membranes, whereas the negative PNPs were internalised mainly by lysosomal endocytosis. Uptake of positive PNPs decreased with increasing steric bulk around the positive charge. A surface charge-specific interaction of clathrin for positive PNPs and caveolin receptors for negative PNPs was observed. These findings confirm that surface charge is important for the cytotoxicity of these PNPs, while they additionally point to considerable additional effects of the steric shielding around positive charges on PNP cytotoxicity.
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