Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 421728
Title Occurrence and properties of bacterial pectate lyases
Author(s) Rombouts, F.M.
Source Landbouwhogeschool Wageningen. Promotor(en): W. Pilnik. - Wageningen : Pudoc - ISBN 9789022004128 - 132
Department(s) Wageningen University
Publication type Dissertation, internally prepared
Publication year 1972
Keyword(s) biochemie - plantkunde - enzymen - enzymologie - fermentatie - fruitgewassen - lyasen - microbiële fysiologie - micro-organismen - fysiologie - groenten - biochemistry - botany - enzymes - enzymology - fermentation - fruit crops - lyases - microbial physiology - microorganisms - physiology - vegetables
Categories Proteins and Enzymes
Abstract <p/>Some 100 pectolytic bacteria belonging to different genera and species, were obtained by isolation from vegetables and by screening of culture collections. The crude enzyme preparations of 19 of these strains were typed by mutual comparison. Differences in the composition of five commercial fungal 'pectinase' preparations were also studied. Purified endo pectate lyase of <em>Arthrobacter</em> which was studied in detail, appeared to attack pectate far 'less randomly', than endo pectate lyases of Bacillus polymyxa or <em>Pseudomonas.</em> The best substrates for pectate lyases were not pectates but 21 to 44% esterified pectins. A new method for the determination of the number average degree of polymerization of pectic substances was introduced. The literature on pectolytic enzymes was reviewed.
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