Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 421731
Title Characterization of pectin lyases on pectins and methyl oligogalacturonates
Author(s) Voragen, A.G.J.
Source Landbouwhogeschool Wageningen. Promotor(en): W. Pilnik. - Wageningen : Pudoc - ISBN 9789022004098 - 121 p.
Department(s) Wageningen University
Publication type Dissertation, internally prepared
Publication year 1972
Keyword(s) lyasen - pectinen - lyases - pectins
Categories Postharvest Treatment / Proteins and Enzymes
Abstract <p/>Pure saturated and unsaturated oligogalacturonic acids, including unsaturated monogalacturonic acid, were isolated and characterized. Their ultraviolet absorbance at 232 run, and their reactivities in the periodate thiobarbituric acid test and carbazole tests were studied. From these compounds methyl oligogalacturonates were prepared, which were used as model substrates.<p/>Pectin lyases (EC 4.2.99.8; poly-α-1,4-D-methyl-galacturonate lyase) were found to be very common in commercial 'pectinase' preparations. From each of three 'pectinase' preparations a pectin lyase was isolated free of other pectolytic enzymes. Their pH optimum, pH stability, isoelectric point and activation energy were determined. Information on the action of the enzymes on pectins with various degrees of esterification ( <em>DE</em> ) was obtained by kinetic studies, by determining the extent of degradation and by analysing the composition of the reaction mixtures. The breakdown mechanism on pectin and pattern of action on methyl oligogalacturonates was studied. Divalent cations were found to activate pectin lyase activity, their activation depended on the pH and <em>DE.</em> Literature on the isolation and characterization of oligogalacturonides and on pectin degrading enzymes is reviewed.
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