Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 421906
Title The effect of endogenous hydrogen peroxide induced by cold treatment in the improvement of tissue regeneration efficiency
Author(s) Szechynska-Hebda, M.; Skrzypek, E.; Dabrowska, G.; Wedzony, M.; Lammeren, A.A.M. van
Source Acta Physiologiae Plantarum 34 (2012)2. - ISSN 0137-5881 - p. 547 - 560.
DOI https://doi.org/10.1007/s11738-011-0852-3
Department(s) Laboratory of Cell Biology
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) oxidative stress - somatic embryogenesis - ascorbate peroxidase - superoxide-dismutase - gene-expression - anther culture - plant-regeneration - winter-wheat - bread wheat - protoplasts
Abstract We propose that oxidative stress resulting from an imbalance between generation and scavenging hydrogen peroxide contributes to tissue regeneration efficiency during somatic embryogenesis of hexaploid winter wheat (Triticum aestivum cv. Kamila) and organogenesis of faba bean (Vicia faba ssp. minor cv. Nadwislanski). Endogenous hydrogen peroxide content and antioxidant capacity of cells were determined in initial explants and callus cultures derived from these explants. Regeneration-competent explants (immature embryos) contained more endogenous H2O2 than explants initiated from regeneration-recalcitrant tissue (mature wheat embryos and faba bean epicotyls). Higher H2O2 levels were observed despite the higher activity of antioxidative enzymes (superoxide dismutase and catalase) and the induction of their gene expression. Calli originating from immature embryos retained the capacity of the initial explants: high H2O2 production was observed during the whole culture period. Low temperature treatment (4°C) was found to be an effective factor, which improved both regeneration ability and H2O2 production. Exogenous application to the medium of H2O2 and catalase blocker (3-aminotriazole), but not FeEDTA and superoxide dismutase blocker (diethyldithiocarbamate), also resulted in the enhancement of regeneration efficiency. These results clearly indicate that plant regeneration is specifically regulated by endogenous H2O2 and by factors, which improve its accumulation. Moreover, a study of the activity of various SOD isoforms suggests that not only the absolute concentration of H2O2, but also its localisation might be responsible for controlling regeneration processes
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