Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 422419
Title MAP Kinase Phosphorylation and cAMP assessment in fungi
Author(s) Mehrabi, R.; M'Barek, S. Ben; Saidi, A.; Abrinbana, M.; Wit, P.J.G.M. de; Kema, G.H.J.
Source In: Plant Fungal Pathogens : Methods and Protocols / Bolton, M.D.D., Thomma, B.P.H.J.P., New York, Dordrecht, Heidelberg, London : Springer (Methods in Molecular Biology 835) - ISBN 9781617795008 - p. 571 - 584.
Department(s) Laboratory of Phytopathology
Biointeracties and Plant Health
PRI BIOINT Moleculair Phytopathology
Publication type Peer reviewed book chapter
Publication year 2012
Abstract The cyclic AMP (cAMP) signaling and mitogen-activated protein (MAP) kinase pathways are the most important signal transduction pathways in eukaryotes. In many plant pathogenic fungi they play pivotal roles in virulence and development. Identifi cation and understanding the role of signal transduction pathways in regulation of cellular responses require robust biochemical techniques. Determination of both the phosphorylation status of MAPKs and the intracellular levels of cAMP is required to unravel the function of these pathways during adaptation of fungi to environmental stress conditions or when particular fungal genes are disrupted or silenced. Here we describe protocols to determine the phosphorylation status of three different MAPKs including Fus3, Slt2 and Hog1 as well as a protocol to measure the intracellular levels of cAMP levels. These protocols can be adapted for a wide range of fungi
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