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Record number 422420
Title Karyotyping Methods for Fungi
Author(s) Mehrabi, R.; Taga, M.; Aghaee, M.; Wit, P.J.G.M. de; Kema, G.H.J.
Source In: Plant Fungal Pathogens : Methods and Protocols / Bolton, M.D.D., Thomma, B.P.H.J.P., New York, Dordrecht, Heidelberg, London : Springer (Methods in Molecular Biology 835) - ISBN 9781617795008 - p. 591 - 602.
Department(s) Laboratory of Phytopathology
PRI BIOINT Moleculair Phytopathology
Publication type Peer reviewed book chapter
Publication year 2012
Abstract Pulsed fi eld gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes
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