Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 422420
Title Karyotyping Methods for Fungi
Author(s) Mehrabi, R.; Taga, M.; Aghaee, M.; Wit, P.J.G.M. de; Kema, G.H.J.
Source In: Plant Fungal Pathogens : Methods and Protocols / Bolton, M.D.D., Thomma, B.P.H.J.P., New York, Dordrecht, Heidelberg, London : Springer (Methods in Molecular Biology 835) - ISBN 9781617795008 - p. 591 - 602.
Department(s) Laboratory of Phytopathology
PRI BIOINT Moleculair Phytopathology
Publication type Peer reviewed book chapter
Publication year 2012
Abstract Pulsed fi eld gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes
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