Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 422573
Title A contribution to the determination of aflatoxin B1, quinine hydrochloride and L(+)-ascorbic acid in foodstuffs by quantitative in situ thin-layer chromatographic analysis
Author(s) Beljaars, P.R.
Source Landbouwhogeschool Wageningen. Promotor(en): W. Pilnik. - Wageningen : Beljaars - 23 p.
Department(s) Wageningen University
Publication type Dissertation, internally prepared
Publication year 1974
Keyword(s) aflatoxinen - karnemelk - chemische samenstelling - voedselinspectie - papierchromatografie - rauwe melk - dunnelaagchromatografie - vitaminen - aflatoxins - buttermilk - chemical composition - food inspection - paper chromatography - raw milk - thin layer chromatography - vitamins
Categories Analytical Chemistry
Abstract <p/>The application of quantitative thin-layer chromatography (TLC) involving in situ spectrodensitometric measurements with a flying-spot densitometer is described in this study for the analysis of aflatoxin B <sub><font size="-1">1</font></sub> , quinine hydrochloride and L(+)-ascorbic acid (vitamin C) in various food and foodstuffs.<p/>Several aspects dealing with the quantitative analysis of aflatoxin B <sub><font size="-1">1</font></sub> in peanuts and peanut products were investigated: isolation, thin-layer chromatographic conditions, visual estimations and fluorodensitometric: measurements. The results of this study contributed to the development of the Official method of analysis for aflatoxin B <sub><font size="-1">1</font></sub> in the Netherlands. A new spotting procedure for aflatoxin assay was introduced ("anti-diagonal" technique) improving the accuracy for visual B <sub><font size="-1">1</font></sub> estiminations for samples with low contamination levels (0-10 μg/kg).<p/>An in situ fluorodensitometric procedure was developed for the quantitative determination of quinine hydrochloride in soft drinks containing quinine whereas in situ densitometric transmittance measurements were applied for the quantitative determination of L(+)-ascorbic acid (vitamin C) in buttermilk.<p/>It was generally concluded that in situ spectrodensitometric procedures were suitable for quantitative routine determinations in food control work, for specific purposes.
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