Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 422859
Title Genetic, morphological and physiological relationships among coryneform bacteria
Author(s) Crombach, W.H.J.
Source Landbouwhogeschool Wageningen. Promotor(en): E.G. Mulder. - Wageningen : Pudoc - ISBN 9789022005286 - 102
Department(s) Wageningen University
Publication type Dissertation, internally prepared
Publication year 1974
Keyword(s) corynebacteriaceae
Categories Bacteria
Abstract <p/>The DNA base composition of the soil arthrobacters tested (65.3 - 67.0% GC) suggests that this group is genetically homogeneous. Hybridization experiments, however, revealed clear differences between the <em>Arthrobacter simplex</em> and the <em>Arthrobacter globiformis</em> strains. The orange cheese coryneforms were fairly homologous, which was shown by the narrow %GC range (63.2 - 63.8, except one strain), the significant hybridization with the type species <em>Brevibacterium linens,</em> and the mutual morphological and physiological resemblance. The majority of the orange sea-fish coryneforms resembled both morphologically and in their GC contents the <em>Brevibacterium linens</em> group; the results of hybridization experiments and physiological studies indicated, however, that only a minority of the sea-fish strains are closely related to the orange cheese coryneforms. With the exception of four strains tested, the majority of the non-orange cheese strains were closely related as concluded from their GC contents (65.5 - 66.9%) and their high degree of hybridization. The soil arthrobacters, the orange cheese and sea-fish coryneforms, and the non-orange cheese coryneforms were found to be only<br/>remotely related because of the poor hybridization between the DNAs of the respective reference strains in addition to differences in GC contents or morphological and physiological characters, or both. In general, the results obtained from DNA analysis and DNA-DNA hybridization experiments and those from morphological and physiological studies were found to be fairly well correlated. A comparative study with native and deep-frozen DNA revealed that freezing at -70 °C and subsequent storing at -21°C for at least half a year had no significant effect on thermal denaturation and hybridization. In the initial renaturation phase, mismatching in hybrids of closely related DNAs was restricted to a few %, and in those of moderately related DNAs to approximately 10%.
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