Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 423317
Title Production of cyanophycin in Rhizopus oryzae through the expression of a cyanophycin synthetase encoding gene
Author(s) Meussen, B.J.; Weusthuis, R.A.; Sanders, J.P.M.; Graaff, L.H. de
Source Applied Microbiology and Biotechnology 93 (2012)3. - ISSN 0175-7598 - p. 1167 - 1174.
Department(s) Systems and Synthetic Biology
Biobased Chemistry and Technology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) nitrogen-containing chemicals - alga anabaena-cylindrica - lactic-acid - ralstonia-eutropha - pseudomonas-putida - aspartic-acid - fumaric-acid - molecular characterization - recombinant strains - biobased production
Abstract Cyanophycin or cyanophycin granule peptide is a protein that results from non-ribosomal protein synthesis in microorganisms such as cyanobacteria. The amino acids in cyanophycin can be used as a feedstock in the production of a wide range of chemicals such as acrylonitrile, polyacrylic acid, 1,4-butanediamine, and urea. In this study, an auxotrophic mutant (Rhizopus oryzae M16) of the filamentous fungus R. oryzae 99-880 was selected to express cyanophycin synthetase encoding genes. These genes originated from Synechocystis sp. strain PCC6803, Anabaena sp. strain PCC7120, and a codon optimized version of latter gene. The genes were under control of the pyruvate decarboxylase promoter and terminator elements of R. oryzae. Transformants were generated by the biolistic transformation method. In only two transformants both expressing the cyanophycin synthetase encoding gene from Synechocystis sp. strain PCC6803 was a specific enzyme activity detected of 1.5 mU/mg protein. In one of these transformants was both water-soluble and insoluble cyanophycin detected. The water-soluble fraction formed the major fraction and accounted for 0.5% of the dry weight. The water-insoluble CGP was produced in trace amounts. The amino acid composition of the water-soluble form was determined and constitutes of equimolar amounts of arginine and aspartic acid
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