Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 423600
Title D-Xylose Concentration-Dependent Hydrolase Expression Profiles and the Function of CreA and XlnR in Aspergillus niger
Author(s) Mach-Aigner, A.R.; Omony, J.; Jovanovic, B.; Boxtel, A.J.B. van; Graaff, L.H. de
Source Applied and Environmental Microbiology 78 (2012)9. - ISSN 0099-2240 - p. 3145 - 3155.
DOI https://doi.org/10.1128/AEM.07772-11
Department(s) Systems and Control Group
Systems and Synthetic Biology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) transcriptional activator xlnr - jecorina trichoderma-reesei - cell-wall polysaccharides - time rt-pcr - hypocrea-jecorina - encoding genes - xylanase expression - beta-xylosidase - enzyme-system - l-arabitol
Abstract Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, d-xylose, is an inducing substance for the coordinate expression of a large number of polysaccharide-degrading enzymes. In this study, the responses of 22 genes to low (1 mM) and high (50 mM) d-xylose concentrations were investigated. These 22 genes encode enzymes that function as xylan backbone-degrading enzymes, accessory enzymes, cellulose-degrading enzymes, or enzymes involved in the pentose catabolic pathway in A. niger. Notably, genes encoding enzymes that have a similar function (e.g., xylan backbone degradation) respond in a similar manner to different concentrations of d-xylose. Although low d-xylose concentrations provoke the greatest change in transcript levels, in particular, for hemicellulase-encoding genes, transcript formation in the presence of high concentrations of d-xylose was also observed. Interestingly, a high d-xylose concentration is favorable for certain groups of genes. Furthermore, the repressing influence of CreA on the transcription and transcript levels of a subset of these genes was observed regardless of whether a low or high concentration of d-xylose was used. Interestingly, the decrease in transcript levels of certain genes on high d-xylose concentrations is not reflected by the transcript level of their activator, XlnR. Regardless of the d-xylose concentration applied and whether CreA was functional, xlnR was constitutively expressed at a low level
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