Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 423698
Title A simple and rapid extraction method for sensitive deterimination of perfluoroalkyl substances in blood serum suitable for exposure evaluation
Author(s) Luque, N.; Ballesteros-Gomez, A.; Leeuwen, S.P.J. van
Source Journal of Chromatography. A, Including electrophoresis and other separation methods 1235 (2012). - ISSN 0021-9673 - p. 84 - 91.
Department(s) RIKILT - R&C Contaminanten
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) tandem mass-spectrometry - organic-compounds prior - solid-phase extraction - liquid-chromatography - perfluorinated compounds - supramolecular solvents - coacervative microextraction - polyfluoroalkyl substances - perfluorooctane sulfonate - human plasma
Abstract In this work, we propose a microextraction method based on a new supramolecular solvent (SUPRAS) made up of reverse aggregates of hexanoic acid, combined with liquid chromatography/triple quadrupole mass spectrometry (LC/QQQ MS-MS) for the determination of the perfluoroalkyl substances (PFASs) in blood serum. A SUPRAS is a nano-structured liquid made up of surfactant aggregates synthesized through a self-assembly process. The method involved the acidification of 765µL of blood serum (600µmol of hydrochloric acid per mL of serum) followed by the addition of hexanoic acid (97µL) and tetrahydrofuran (THF) (600µL), conditions under which the supramolecular solvent (~360µL) formed in situ after vortex-shaking and centrifugation. Parameters affecting extraction efficiency and concentration factors were studied. The overall sample treatment took only 20min and several samples (20-30) can be simultaneously analyzed using conventional lab equipments, making additional investments unnecessary. Recoveries for the internal standards in samples ranged from 75 to 89% with relative standard deviations between 1 and 15%. Calibration was based on the use of internal standards. The method was very sensitive with detection limits ranging from 2 to 20pgmL(-1) for PFASs. The approach developed was successfully applied to the determination of PFASs in different blood serum samples. The concentration of PFASs found in samples of animal origin ranged between 17 and 197.3pgmL(-1) and between 84 and 5168pgmL(-1) in samples of human origin. Both the analytical and operational features of this method make it suitable for the evaluation of exposure to PFASs.
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