Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 424070
Title A Multi-Platform Flow Device for Microbial (Co-) Cultivation and Microscopic Analysis
Author(s) Hesselman, M.C.; Odoni, D.I.; Ryback, B.M.; Groot, S. de; Heck, R.G.A. van; Keijsers, J.; Kolkman, P.; Nieuwenhuijse, D.; Nuland, Y.M.; Sebus, E.; Spee, R.; Vries, H. de; Wapenaar, M.T.; Ingham, C.J.; Schroen, K.; Martins Dos Santos, V.A.P.; Spaans, S.K.; Hugenholtz, F.; Passel, M.W.J. van
Source PLoS One 7 (2012)5. - ISSN 1932-6203
Department(s) Microbiological Laboratory
Food Process Engineering
Systems and Synthetic Biology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) culture - microorganisms - population - resistance - membranes - bacteria - chamber - support - arrays
Abstract Novel microbial cultivation platforms are of increasing interest to researchers in academia and industry. The development of materials with specialized chemical and geometric properties has opened up new possibilities in the study of previously unculturable microorganisms and has facilitated the design of elegant, high-throughput experimental set-ups. Within the context of the international Genetically Engineered Machine (iGEM) competition, we set out to design, manufacture, and implement a flow device that can accommodate multiple growth platforms, that is, a silicon nitride based microsieve and a porous aluminium oxide based microdish. It provides control over (co-)culturing conditions similar to a chemostat, while allowing organisms to be observed microscopically. The device was designed to be affordable, reusable, and above all, versatile. To test its functionality and general utility, we performed multiple experiments with Escherichia coli cells harboring synthetic gene circuits and were able to quantitatively study emerging expression dynamics in real-time via fluorescence microscopy. Furthermore, we demonstrated that the device provides a unique environment for the cultivation of nematodes, suggesting that the device could also prove useful in microscopy studies of multicellular microorganisms
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