Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424161
Title Growth and lipid production of Umbelopsis isabellina on a solid substrate - Mechanistic modeling and validation
Author(s) Meeuwse, P.; Klok, A.J.; Haemers, S.; Tramper, J.; Rinzema, A.
Source Process Biochemistry 47 (2012)8. - ISSN 1359-5113 - p. 1228 - 1242.
DOI https://doi.org/10.1016/j.procbio.2012.04.017
Department(s) Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) state fermentation system - gamma-linolenic acid - oleaginous fungi - diffusion-coefficients - biodiesel production - aspergillus-oryzae - chemostat model - oxygen - glucose - accumulation
Abstract Microbial lipids are an interesting feedstock for biodiesel. Their production from agricultural waste streams by fungi cultivated in solid-state fermentation may be attractive, but the yield of this process is still quite low. In this article, a mechanistic model is presented that describes growth, lipid production and lipid turnover in a culture of Umbelopsis isabellina on ¿-carrageenan plates containing the monomers glucose and alanine as C-source and N-source, respectively, and improves the understanding of the complex solid-state system. The model includes reaction kinetics and diffusion of glucose, alanine and oxygen. It is validated empirically and describes the different phases of the culture very well: exponential growth, linear growth because of oxygen limitation, accumulation of lipids and carbohydrates after local N-depletion and turnover of lipids after local C-depletion. Extending the model with an unidentified extracellular product improved the fit of the model to the data. The model shows that oxygen limitation is extremely important in solid-state cultures using monomers, and explains the difference in production rate with submerged cultures. However, the results also show that the specific lipid production rate in solid-state cultures is much lower than in submerged cultures, which results in a low lipid yield
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