Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424276
Title Validation of reference genes for real-time quantitative PCR normalisation in non-heading Chinese cabbage
Author(s) Xiao, D.; Zhang, N.; Jianjun Zhao, Jianjun; Bonnema, A.B.; Hou, X.L.
Source Functional Plant Biology 39 (2012)4. - ISSN 1445-4408 - p. 342 - 350.
DOI https://doi.org/10.1071/FP11246
Department(s) Laboratory of Plant Breeding
EPS
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) housekeeping genes - internal control - brassica-rapa - expression - rice - identification - arabidopsis - selection - stress
Abstract Non-heading Chinese cabbage is an important vegetable crop that includes pak choi, caixin and several Japanese vegetables like mizuna, mibuna and komatsuna. Gene expression studies are frequently used to unravel the genetics of complex traits and in such studies the proper selection of reference genes for normalisation is crucial. We assessed the expression of 13 candidate reference genes including ACTIN, ACTIN-1, ACTIN-2, GAPDH, Tub-a, CyP, EF1-a, 18S rRNA, UBQ, UBC30, PPR, PP2A and MDH. Their expression stabilities were analysed using two programs, geNorm and NormFinder, in 20 different samples that represent four strategic groups. Results showed that no single gene was uniformly expressed in all tested samples. ACTIN and CyP are proposed as good reference genes when studying developmental stages. CyP, Tub-a and UBC30 are good reference genes when studying different tissues (from flowering to seed set). CyP and Tub-a are the most stable reference genes under biotic stress treatments using the fungi Peronospora parasitica and Alternaria brassicicola. UBC30, EF1-a and ACTIN are recommended for normalisation in abiotic stress studies, including hormone, salt, drought, cold and heath treatments. Moreover, at least five reference genes (ACTIN, CyP, UBC30, EF1-a and UBQ) are required for accurate q RTPCR data normalisation when studying gene expression across all tested samples
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