Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424309
Title The influence of casein and urea as nitrogen sources on in vitro equine caecal fermentation
Author(s) Santos, A.S.; Ferreira, L.M.M.; Martin-Rosset, W.; Cotovio, M.; Silva, F.; Bennett, R.N.; Cone, J.W.; Bessa, R.J.B.; Rodrigues, M.A.M.
Source Animal 6 (2012)7. - ISSN 1751-7311 - p. 1096 - 1102.
Department(s) Animal Nutrition
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) rumen microbial-growth - gas-production - amino-acids - protein - ponies - digestibility - metabolism - peptides - kinetics - invitro
Abstract To access the fermentative response of equine caecal microbial population to nitrogen availability, an in vitro study was conducted using caecal contents provided with adequate energy sources and nitrogen as limiting nutrient. Two nitrogen (N) sources were provided, protein (casein) and non-protein (urea). Caecal fluid, taken from three cannulated horses receiving a hay–concentrate diet, was mixed with a N-free buffer–mineral solution. The influence of four N levels (3.7, 6.3, 12.5 or 25 mg of N in casein or urea) was studied using the gas production technique. Total volatile fatty acids (VFA), NH3-N and gas production were measured after a 24-h incubation period. Microbial biomass was estimated using adenine and guanine bases as internal markers, and ATP production was estimated stoichiometrically. Microbial growth efficiency (YATP) and gas efficiency (Egas) were estimated. Fermentation with casein as the sole N source was generally characterized by lower total VFA, NH3-N, total gas production and higher acetate : propionate (A : P) ratio and YATP than with urea. Results herein presented indicate that, under these in vitro conditions, caecal microbial population does in fact use urea N, but less efficiently than casein in terms of microbial growth
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