Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424492
Title Streamlining genomes: toward the generation of simplified and stabilized microbial systems
Author(s) Leprince, A.; Passel, M.W.J. van; Martins Dos Santos, V.A.P.
Source Current Opinion in Biotechnology 23 (2012)5. - ISSN 0958-1669 - p. 651 - 658.
DOI https://doi.org/10.1016/j.copbio.2012.05.001
Department(s) Systems and Synthetic Biology
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) engineered escherichia-coli - reduced-genome - advanced biofuels - gene replacement - bacteria - design - expression - evolution - growth - host
Abstract At the junction between systems and synthetic biology, genome streamlining provides a solid foundation both for increased understanding of cellular circuitry, and for the tailoring of microbial chassis towards innovative biotechnological applications. Iterative genomic deletions (targeted and random) helps to generate simplified, stabilized and predictable genomes, whereas multiplexing genome engineering reveals a broad functional genetic diversity. The decrease in oligo and gene synthesis costs promises effective combinatorial tools for the generation of chassis based on streamlined and tractable genomes. Here we review recent progresses in streamlining genomes through recombineering techniques aiming to generate insights into cellular mechanisms and responses towards the design and assembly of streamlined genome chassis together with new cellular modules in diverse biotechnological applications
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