Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424981
Title Bead-based suspension array for simultaneous detection of antibodies against the Rift Valley fever virus nucleocapsid and Gn glycoprotein
Author(s) Wal, F.J. van der; Achterberg, R.P.; Boer, S.M. de; Boshra, H.; Brun, A.; Maassen, C.B.M.; Kortekaas, J.A.
Source Journal of Virological Methods 183 (2012)2. - ISSN 0166-0934 - p. 99 - 105.
Department(s) CVI Infection Biology
CVI - Division Virology
CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) linked-immunosorbent-assay - domestic ruminants - indirect elisa - igm antibodies - capture elisa - nss protein - n-protein - humans - validation - sandwich
Abstract A multiplex bead-based suspension array was developed that can be used for the simultaneous detection of antibodies against the surface glycoprotein Gn and the nucleocapsid protein N of Rift Valley fever virus (RVFV) in various animal species. The N protein and the purified ectodomain of the Gn protein were covalently linked to paramagnetic Luminex beads. The performance of the resulting multiplex immunoassay was evaluated by testing a comprehensive and well-characterized panel of sera from sheep, cattle and humans. The suitability of this multiplex immunoassay to differentiate infected from vaccinated animals (DIVA) was investigated by testing sera from lambs vaccinated with a paramyxovirus vaccine vector expressing the RVFV surface glycoproteins Gn and Gc. The results suggest that the bead-based suspension array can be used as a DIVA assay to accompany several recently developed experimental vaccines that are based on RVFV glycoproteins, and are devoid of the N protein.
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