Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 424982
Title Genetic Features Differentiating Bovine, Food, and Human Isolates of Shiga Toxin-Producing Escherichia coli O157 in The Netherlands
Author(s) Franz, E.; Hoek, A.H.A.M. van; Wal, F.J. van der; Boer, A.G. de; Zwartkruis-Nahuis, A.; Zwaluw, K. van der; Aarts, H.J.M.; Heuvelink, A.E.
Source Journal of Clinical Microbiology 50 (2012)3. - ISSN 0095-1137 - p. 772 - 780.
DOI http://dx.doi.org/10.1128/JCM.05964-11
Department(s) RIKILT - Business Unit Safety & Health
RIKILT - V&G Microbiologie & Novel Foods
CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) genotypic characterization - virulence factors - human infection - strain origin - dairy-cattle - prevalence - expression - lineages - vtec - identification
Abstract The frequency of Escherichia coli O157 genotypes among bovine, food, and human clinical isolates from The Netherlands was studied. Genotyping included the lineage-specific polymorphism assay (LSPA6), the Shiga-toxin-encoding bacteriophage insertion site assay (SBI), and PCR detection and/or subtyping of virulence factors and markers [stx1, stx2a/stx2c, q21/Q933, tir(A255T), and rhsA(C3468G)]. LSPA6 lineage II dominated among bovine isolates (63%), followed by lineage I/II (35.6%) and lineage I (1.4%). In contrast, the majority of the human isolates were typed as lineage I/II (77.6%), followed by lineage I (14.1%) and lineage II (8.2%). Multivariate analysis revealed that the tir(A255T) SNP and the stx2a/stx2c gene variants were the genetic features most differentiating human from bovine isolates. Bovine and food isolates were dominated by stx2c (86.4% and 65.5%, respectively). Among human isolates, the frequency of stx2c was 36.5%, while the frequencies of stx2a and stx2a plus stx2c were 41.2% and 22.4%, respectively. Bovine isolates showed equal distribution of tir(255A) (54.8%) and tir(255T) (45.2%), while human isolates were dominated by the tir(255T) genotype (92.9%). LSPA6 lineage I isolates were all genotype stx2c and tir(255T), while LSPA6 lineage II was dominated by tir(255A) (86.4%) and stx2c (90.9%). LSPA6 lineage I/II isolates were all genotype tir(255T) but showed more variation in stx2 types. The results support the hypothesis that in The Netherlands, the genotypes primarily associated with human disease form a minor subpopulation in the bovine reservoir. Comparison with published data revealed that the distribution of LSPA6 lineages among bovine and human clinical isolates differs considerably between The Netherlands and North America
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.