Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424985
Title Prevalence and characteristics of quinolone resistance in Escherichia coli in veal calves
Author(s) Hordijk, J.; Veldman, K.T.; Dierikx, C.M.; Essen-Zandbergen, A. van; Wagenaar, J.A.; Mevius, D.J.
Source Veterinary Microbiology 156 (2012)1-2. - ISSN 0378-1135 - p. 136 - 142.
Department(s) CVI Bacteriology and Epidemiology
ID - Infectieziekten
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) efflux pump inhibitor - salmonella-enterica - qnr genes - fluoroquinolone resistance - antibiotic-resistance - european countries - modifying enzyme - clinical isolate - plasmid - mechanisms
Abstract Quinolone resistance is studied and reported increasingly in isolates from humans, food-producing animals and companion animals. Resistance can be caused by chromosomal mutations in topoisomerase genes, plasmid-mediated resistance genes, and active transport through efflux pumps. Cross sectional data on quinolone resistance mechanisms in non-pathogenic bacteria from healthy veal calves is limited. The purpose of this study was to determine the prevalence and characteristics of quinolone resistance mechanisms in Escherichia coli isolates from veal calves, after more than 20 years of quinolone usage in veal calves. MIC values were determined for all isolates collected as part of a national surveillance program on antimicrobial resistance in commensal bacteria in food-producing animals in The Netherlands. From the strains collected from veal calves in 2007 (n = 175) all isolates with ciprofloxacin MIC = 0.125 mg/L (n = 25) were selected for this study, and screened for the presence of known quinolone resistance determinants. In this selection only chromosomal mutations in the topoisomerase type II and IV genes were detected. The number of mutations found per isolate correlated with an increasing ciprofloxacin MIC. No plasmid-mediated quinolone resistance genes were found. The contribution of efflux pumps varied from no contribution to a 16-fold increase in susceptibility. No correlation was found with the presence of resistance genes of other antimicrobial classes, even though all quinolone non-wild type isolates were resistant to 3 or more classes of antibiotics other than quinolones. Over twenty years of quinolone usage in veal calves in The Netherlands did not result in a widespread occurrence of plasmid-mediated quinolone resistance, limiting the transmission of quinolone resistance to clonal distribution
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