Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424986
Title Occurrence and characteristics of extended-spectrum-ß-lactamase- and AmpC-producing clinical isolates derived from companion animals and horses
Author(s) Dierikx, C.M.; Duijkeren, E. van; Schoormans, A.; Essen-Zandbergen, A. van; Veldman, K.T.; Kant, A.; Huijsdens, X.; Zwaluw, K. van der; Wagenaar, J.A.; Mevius, D.J.
Source Journal of Antimicrobial Chemotherapy 67 (2012)6. - ISSN 0305-7453 - p. 1368 - 1374.
DOI https://doi.org/10.1093/jac/dks049
Department(s) CVI Bacteriology and Epidemiology
ID - Infectieziekten
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) mediated quinolone resistance - escherichia-coli - salmonella-enterica - beta-lactamases - antimicrobial resistance - genes - enterobacteriaceae - cephalosporins - identification - epidemiology
Abstract To investigate the occurrence and characteristics of extended-spectrum -lactamase (ESBL)- and AmpC-producing Enterobacteriaceae isolates in clinical samples of companion animals and horses and compare the results with ESBL/AmpC-producing isolates described in humans. Between October 2007 and August 2009, 2700 Enterobacteriaceae derived from clinical infections in companion animals and horses were collected. Isolates displaying inhibition zones of 25 mm for ceftiofur and/or cefquinome by disc diffusion were included. ESBL/AmpC production was confirmed by combination disc tests. The presence of resistance genes was identified by microarray, PCR and sequencing, Escherichia coli genotypes by multilocus sequence typing and antimicrobial susceptibility by broth microdilution. Sixty-five isolates from dogs (n38), cats (n14), horses (n12) and a turtle were included. Six Enterobacteriaceae species were observed, mostly derived from urinary tract infections (n32). All except 10 isolates tested resistant to cefotaxime and ceftazidime by broth microdilution using clinical breakpoints. ESBL/AmpC genes observed were bla(CTX-M-1, -2, -9, -14, -15,) bla(TEM-52), bla(CMY-2) and bla(CMY-39). bla(CTX-M-1) was predominant (n17). bla(CTX-M-9) occurred in combination with qnrA1 in 3 of the 11 Enterobacter cloacae isolates. Twenty-eight different E. coli sequence types (STs) were found. E. coli carrying bla(CTX-M-1) belonged to 13 STs of which 3 were previously described in Dutch poultry and patients. This is the first study among a large collection of Dutch companion animals and horses characterizing ESBL/AmpC-producing isolates. A similarity in resistance genes and E. coli STs among these isolates and isolates from Dutch poultry and humans may suggest exchange of resistance between different reservoirs.
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