Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 424988
Title Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles
Author(s) Harmsen, M.M.; Fijten, H.P.D.; Westra, D.F.; Coco-Martin, J.M.
Source Vaccine 29 (2011)15. - ISSN 0264-410X - p. 2682 - 2690.
DOI https://doi.org/10.1016/j.vaccine.2011.01.069
Department(s) CVI Virology
CVI Infection Biology
ASG ProductenProducten
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) domain antibody fragments - whole virus-particles - monoclonal-antibodies - thermal-stability - passive-immunization - international bank - protein subunit - sandwich elisa - field strains - vaccine
Abstract Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain antibody fragments that bind specifically to either 146S or 12S particles we developed two ELISAs for the quantification of these particles in FMDV antigen preparations used for vaccine manufacturing. Only O serotype strains are detected in the 146S specific ELISA whereas strains of most serotypes are detected in the 12S specific ELISA. However, the 146S concentration of A and Asia 1 serotype strains could be measured indirectly using the 12S specific ELISA by prior conversion of 146S into 12S particles by heat treatment. This allowed us to demonstrate that addition of the preservative thiomersal to FMDV antigens stimulates the dissociation into 12S particles of O, A and Asia 1 serotype strains upon prolonged storage at 4 °C. FMDV dissociation is known to result in a strongly reduced immunogenicity, which was experimentally confirmed here. Therefore, we recommend to omit thiomersal from FMD vaccines to increase its shelf life
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