Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 425231
Title Effect-Directed Assessment of the Bioaccumulation Potential and Chemical Nature of Ah Receptor Agonists in Crude and Refined Oils
Author(s) Vrabie, C.M.; Sinnige, T.L.; Murk, A.J.; Jonker, M.T.O.
Source Environmental Science and Technology 46 (2012)3. - ISSN 0013-936X - p. 1572 - 1580.
Department(s) IMARES
Sub-department of Toxicology
Aquatic Ecology and Water Quality Management
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) polycyclic aromatic-hydrocarbons - in-vitro toxicity - petroleum-products - contaminated sediment - mediated responses - water discharges - heavy oil - assays - bioavailability - identification
Abstract Recent studies have indicated that in addition to narcosis certain chemicals in crude oils and refined petroleum products may induce specific modes of action, such as aryl hydrocarbon receptor (AhR) agonism. The risks these toxic compounds pose to organisms depend on internal exposure levels, as driven by the chemicals’ bioaccumulation potential. Information on this potential however is lacking, as the chemicals’ identity mostly is unknown. This study showed that AhR agonists bioaccumulate from oil-spiked sediments into aquatic worms and persist in the worms for at least several weeks. Chemical fractionations of eight pure oils into saturates, aromatics, resins, and asphaltenes (SARA), followed by effect-directed analyses using in vitro reporter gene assays revealed that the agonists predominantly are aromatic and resin-like chemicals. Some of the compounds were easily metabolized in vitro, while others were resistant to biotransformation. HPLC-assisted hydrophobicity profiling subsequently indicated that the AhR-active chemicals had a high to extremely high bioaccumulation potential, considering their estimated logKow values of 4 to >10. Most of the AhR agonism, however, was assigned to compounds with logKow of 5–8. These compounds were present mainly in the mid to high boiling point fractions of the oils (C14–C32 alkane range), which are usually not being considered (the most) toxic in current risk assessment. The fractionations further revealed considerable oil and fraction-dependent antagonism in pure oils and SARA fractions. The results of this study clearly demonstrate that crude oils and refined petroleum products contain numerous compounds that can activate the AhR and which because of their likely persistence and extremely high bioaccumulation potential could be potential PBT (persistent, bioaccumulative and toxic) or vPvB (very persistent and very bioaccumulative) substance candidates. Many chemicals were identified by GC-MS, but the responsible individual compounds could not be exactly identified in the complex mixtures of thousands of compounds. Because this obstructs a classical PBT risk assessment, our results advocate an adapted risk assessment approach for complex mixtures in which low concentrations of very potent compounds are responsible for mixture effects.
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