In livestock houses, particulate matter (PM) and airborne microorganism are two of the most relevant air pollutants. Particulate matter may carry microorganisms, the inhalation of which can cause detrimental health effects. The aim of this study was to study the spatial distribution of airborne aerobic mesophilic bacteria in the air of a broiler farm (rearing poultry for meat production), to quantify the concentration of airborne aerobic mesophilic bacteria and PM in the air and to study their evolution in time, as well as to evaluate the relationship between particle size and airborne aerobic mesophilic bacteria, and to correlate the evolution of PM concentrations and aerobic mesophilic bacteria concentrations. For this purpose, the air of two broiler rooms in an experimental broiler farm was sampled weekly using different methods during 42 days. There was a spatial gradient (p
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