Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 425306
Title Determination of Fumonisins B-1 and B-2 in Corn-Based Foods for Infants and Young Children by LC with Immunoaffinity Column Cleanup: Interlaboratory Validation Study
Author(s) Solfrizzo, M.; Girolamo, A. De; Gambacorta, L.; Visconti, A.; Stroka, J.; Egmond, H.P. van
Source Journal of AOAC International 94 (2011)3. - ISSN 1060-3271 - p. 900 - 908.
Department(s) RIKILT - R&C Natuurlijke Toxinen en Pesticiden
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) extraction - performance - precision - products
Abstract A liquid chromatographic method for the determination of fumonisins B-1 (FB1) and B-2 (FB2) in corn-based foods for infants and young children was subjected to an interlaboratory validation study involving 11 laboratories. Five blind duplicate sample pairs of each matrix were analyzed to establish the accuracy, repeatability, and reproducibility of the method. Mass fractions in the baby food samples ranged from 89.1 to 384.4 mu g/kg FB1 and from 22.5 to 73.6 mu g/kg FB2. The method involved a warm extraction with citrate phosphate buffer-methanol-acetonitrile (50 + 25 + 25, v/v/v), a cleanup through an immunoaffinity column, and an end-determination of fumonisins by LC after automated precolumn derivatization with o-phthaldialdehyde reagent. RSDs for within-laboratory repeatability (RSDr) ranged from 6.8 to 23.5% for FB1 and 7.6 to 22.9% for FB2. RSDs for between-laboratory reproducibility (RSDR) ranged from 15.4 to 26.2% for FB1 and 21.6 to 36.3% for FB2. Mean FB1 recoveries from baby foods spiked at 100.0 and 250.0 mu g/kg were 89 and 96%, respectively; for FB2 spiked foods at 25.0 and 62.5 mu g/kg recoveries were 90 and 85%, respectively. HorRat values ranged from 0.8 to 1.2 for FB1, whereas for FB2 they ranged from 0.9 to 1.4 when calculated according to Horwitz, and from 1.0 to 1.7 when calculated according to Thompson, indicating an acceptable among-laboratory precision for all matrixes (HorRat values
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