Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 425347
Title Molecular Diagnostics for Complex Pest and Disease Resistance and Tuber Quality Traits: Concept, Achievements and Perspectives
Author(s) Gebhardt, C.; Urbany, C.; Li, L.; Stich, B.; Caldas Paulo, M.J.; Draffehn, A.; Ballvora, A.
Source Potato Research 54 (2011)4. - ISSN 0014-3065 - p. 313 - 318.
DOI https://doi.org/10.1007/s11540-011-9193-2
Department(s) Biometris (PPO/PRI)
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) late blight - potato - organization - association - genome - gene
Abstract We summarize the concept of molecular diagnostic of complex traits related to pest and disease resistance and to tuber quality of potato, and describe recent achievements and perspectives. Many potato characteristics are controlled by multiple genetic and environmental factors. Knowing the genes and their allelic variants that underlay these characteristics allows developing molecular diagnostic tools to select for improved potato cultivars. Diagnostic DNA-based markers can be used to identify superior genotypes (precision breeding). Diagnostic markers can be identified by combining quantitative trait locus mapping, candidate gene mapping and association mapping using functional and positional candidate genes as markers. This approach was successfully used to identify loci, which contribute to the natural variation of important agronomic traits, including resistance against root cyst nematodes, late blight and wart disease and tuber quality (resistance to bruising and chip colour). In the future, whole genome association mapping based on single-nucleotide polymorphism genotyping methods in combination with the annotated potato genome sequence will allow identifying additional genes and gene variants controlling agronomic performance in potato. Prerequisites are accurate phenotyping under field conditions of advanced breeding materials, cost-effective and reliable genome-wide genotyping methods, and user-friendly software tools allowing to extract knowledge from massive quantities of data. This will further facilitate molecular diagnosis, selection and combination of superior alleles in potato-breeding programmes.
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