Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 425384
Title Genetic mapping and characterization of the globe artichoke (+)-germacrene A synthase gene, encoding the first dedicated enzyme for biosynthesis of the bitter sesquiterpene lactone cynaropicrin
Author(s) Menin, B.; Comino, C.; Portis, E.; Moglia, A.; Cankar, K.; Bouwmeester, H.J.; Lanteri, S.; Beekwilder, M.J.
Source Plant Science 190 (2012). - ISSN 0168-9452 - p. 1 - 8.
DOI https://doi.org/10.1016/j.plantsci.2012.03.006
Department(s) PRI BIOS Applied Metabolic Systems
Laboratory of Plant Physiology
EPS-3
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) germacrene-a synthase - cynara-cardunculus l - functional-characterization - artemisia-annua - cdna isolation - leaf extract - scolymus l. - chicory - hydroxycinnamoyltransferase - identification
Abstract Globe artichoke (Cynara cardunculus var. scolymus L., Asteraceae) is a perennial crop traditionally consumed as a vegetable in the Mediterranean countries and rich in nutraceutically and pharmaceutically active compounds, including phenolic and terpenoid compounds. Its bitter taste is caused by its high content of sesquiterpene lactones (STLs), such as cynaropicrin. The biosynthetic pathway responsible for STL biosynthesis in globe artichoke is unknown, but likely proceeds through germacrene A, as has been shown for other Asteraceae species. Here, we investigated the accumulation of cynaropicrin in different tissues of globe artichoke, and compared it to accumulation of phenolic compounds. Cynaropicrin concentration was highest in old leaves. A putative germacrene A synthase (GAS) gene was identified in a set of ~19,000 globe artichoke unigenes. When heterologously expressed in Escherichia coli, the putative globe artichoke GAS converted farnesyl diphosphate (FPP) into (+)-germacrene A. Among various tissues assayed, the level of globe artichoke GAS expression was highest in mature (six week old) leaves. A sequence polymorphism within a mapping population parent allowed the corresponding GAS gene to be positioned on a genetic map. This study reports the isolation, expression and mapping of a key gene involved in STL biosynthesis in C. cardunculus. This is a good basis for further investigation of this pathway
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