Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 425887
Title Identification of HR-inducing cDNAs from plant pathogens via a Gateway-compatible binary Potato Virus X-expression vector
Author(s) Esse, H.P. van
Source In: Plant Fungal Pathogens: Methods and Protocols / Bolton, M.D., Thomma, B.P.H.J., New York, USA : Humana Press, c/o Springer Science+Business Media (Methods in Molecular Biology 835) - ISBN 9781617795008 - p. 97 - 105.
DOI https://doi.org/10.1007/978-1-61779-501-5_6
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Peer reviewed book chapter
Publication year 2012
Abstract Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.
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