Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 425891
Title A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens
Author(s) Tameling, W.I.L.
Source In: Plant Fungal Pathogens: Methods and Protocols / Bolton, M.D., Thomma, B.P.H.J., New York, USA : Humana Press, c/o Springer Science+Business Media (Methods in Molecular Biology 835) - ISBN 9781617795008 - p. 585 - 590.
DOI http://dx.doi.org/10.1007/978-1-61779-501-5_36
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Peer reviewed book chapter
Publication year 2012
Abstract Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms. Their low expression makes it challenging to purify these receptors from plants in sufficient quantities to be able to identify interacting proteins by mass spectrometry. Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.
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