Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 425893
Title Isolation of apoplastic fluid from leaf tissue by the vacuum infiltration-centrifugation technique
Author(s) Joosten, M.H.A.J.
Source In: Plant Fungal Pathogens: Methods and Protocols / Bolton, M.D., Thomma, B.P.H.J., New York, USA : Humana Press, c/o Springer Science+Business Media (Methods in Molecular Biology 835) - ISBN 9781617795008 - p. 603 - 610.
DOI https://doi.org/10.1007/978-1-61779-501-5_38
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Peer reviewed book chapter
Publication year 2012
Abstract Upon infection of plants by pathogens, at least at the early stages of infection, the interaction between the two organisms occurs in the apoplast. To study the molecular basis of host susceptibility vs. resistance on the one hand, and pathogen virulence vs. avirulence on the other, the identification of extracellular compounds such as pathogen effectors that determine the outcome of the interaction is essential. Here, I describe the vacuum infiltration-centrifugation technique, which is an extremely simple and straightforward method to explore one of the most important battlefields of a plant–pathogen interaction; the apoplast.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.