Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 426145
Title Effect of heat treatment on the potential allergenicity and conformational structure of egg allergen ovotransferrin
Author(s) Tong, P.; Chen, H.; Gao, J.; Li, X.; Jian, S.; Wichers, H.J.; Wu, Z.; Yang, A.
Source Food Chemistry 131 (2012). - ISSN 0308-8146 - p. 603 - 610.
Department(s) FBR Fresh Supply Chains
Food Chemistry Group
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) protein isolate - white proteins - food allergy - antigenicity - lactoglobulin - management - stability - children
Abstract The aim of this research was to assess heat induced changes of the potential allergenicity and the structure of ovotransferrin (OVT). Changes in conformational structure of OVT were characterised by circular dichroism (CD) spectroscopy, ultraviolet (UV) absorption spectroscopy and fluorescence spectroscopy. A reversible unfolding structure was found at 55–60 C, followed by the loss of secondary structure at 70–80 C. Cleavage and rearrangements of disulphide bonds occurred when heated above 80 C. The indirect enzyme-linked immunosorbent assay (ELISA) of heat-treated OVT with egg allergic patients’ sera and specific rabbit polyclonal antibodies against native OVT showed that the potential allergenicity of OVT correlated well with the structural changes. Allergenicity increased with the unfolding of its structure, and decreased with the cleavage of disulphide bonds and the rearrangements of disulphide bonds. The results suggested that conformational changes of OVT induced by heat treatment significantly influenced the allergenicity of OVT, and the potential allergenicity of heated OVT is related to individual patient characteristics.
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