Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 426650
Title Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga
Author(s) Lamers, P.P.; Janssen, M.; Vos, R.C.H. de; Bino, R.J.; Wijffels, R.H.
Source Journal of Biotechnology 162 (2012)1. - ISSN 0168-1656 - p. 21 - 27.
Department(s) Bioprocess Engineering
PRI BIOS Applied Metabolic Systems
Laboratory of Plant Physiology
AFSG Staff Departments (FBR)
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) beta-carotene - biochemical-composition - nutrient limitation - low-temperature - high light - accumulation - bardawil - alga - induction - stress
Abstract Nitrogen availability and light intensity affect ß-carotene overproduction in the green alga Dunaliella salina. Following a previous study on high-light stress, we here report on the effect of nitrogen depletion on the growth characteristics and ß-carotene as well as fatty acid metabolism of D. salina under a constant light regime in a turbidostat. Upon nitrogen depletion, the biomass yield on absorbed light approximately doubled, due to a transient increase in cell division rate, swelling of the cells and a linear increase of the density of the cells. Simultaneously, ß-carotene started to accumulate up to a final intracellular concentration of 14 mg LCV-1 (i.e. 2.7% of AFDW). This ß-carotene production accounted for 6% of the increased density of the cells, indicating that other biochemical constituents accumulated as well. Since D. salina accumulates ß-carotene in lipid globules, we also determined the fatty acid content and composition of D. salina. The intracellular concentration of the total fatty acid pool did not change significantly during nitrogen starvation, indicating that ß-carotene and total fatty acid accumulation were unrelated, similar to what was found previously for high-light treated cells. However, for both high-light and nitrogen stress, ß-carotene accumulation negatively correlated with the degree of unsaturation of the total fatty acid pool and, within the individual fatty acids, correlated positively with oleic acid biosynthesis, suggesting that oleic acid may be a key component of the lipid-globule-localized triacylglycerols and thereby in ß-carotene accumulation.
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