Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 426682
Title Characterization of a spore-specific protein of the Bacillus cereus group
Author(s) From, C.; Voort, M. van der; Abee, T.; Granum, P.E.
Source FEMS Microbiology Letters 331 (2012)2. - ISSN 0378-1097 - p. 152 - 159.
DOI http://dx.doi.org/10.1111/j.1574-6968.2012.02562.x
Department(s) Laboratory of Phytopathology
Food Microbiology Laboratory
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) gram-positive bacteria - low-gc-content - anthracis exosporium - nucleotide-sequence - sigma-factors - subtilis - surface - outer - tonb - germination
Abstract Bc1245 is a monocistronic chromosomal gene of Bacillus cereus ATCC 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in B. cereus. Bc1245 is highly conserved in the genome of members of the B. cereus group, indicating an important function of the gene in this group of bacteria. Quantitative PCR revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell–specific transcription factor sK. The sK regulon includes structural components of the spore (such as coat proteins), and it is therefore plausible that bc1245 might encode a structural outer spore protein. This was confirmed by detection of BC1245 in exosporium extracts from B. cereus by immunoblotting against BC1245 antiserum.
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