Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 426903
Title Functional analysis of the role of CggR (central glycolytic gene regulator) in Lactobacillus plantarum by transcriptome analysis
Author(s) Rud, I.; Naterstad, K.; Bongers, R.S.; Molenaar, D.; Kleerebezem, M.; Axelsson, L.
Source Microbial Biotechnology 4 (2011)3. - ISSN 1751-7907 - p. 345 - 356.
DOI http://dx.doi.org/10.1111/j.1751-7915.2010.00223.x
Department(s) Microbiological Laboratory
Host Microbe Interactomics
VLAG
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) carbon catabolite repression - complete genome sequence - control protein ccpa - lactococcus-lactis - bacillus-subtilis - pyruvate oxidase - listeria-monocytogenes - enterococcus-faecalis - mesentericin y105 - metabolic fluxes
Abstract P>The level of the central glycolytic gene regulator (CggR) was engineered in Lactobacillus plantarum NC8 and WCFS1 by overexpression and in-frame mutation of the cggR gene in order to evaluate its regulatory role on the glycolytic gap operon and the glycolytic flux. The repressor role of CggR on the gap operon was indicated through identification of a putative CggR operator and transcriptome analysis, which coincided with decreased growth rate and glycolytic flux when cggR was overexpressed in NC8 and WCFS1. The mutation of cggR did not affect regulation of the gap operon, indicating a more prominent regulatory role of CggR on the gap operon under other conditions than tested (e.g. fermentation of other sugars than glucose or ribose) and when the level of the putative effector molecule FBP is reduced. Interestingly, the mutation of cggR had several effects in NC8, i.e. increased growth rate and glycolytic flux and regulation of genes with functions associated with glycerol and pyruvate metabolism; however, no effects were observed in WCFS1. The affected genes in NC8 are presumably regulated by CcpA, since putative CRE sites were identified in their upstream regions. The interconnection with CggR and CcpA-mediated control on growth and metabolism needs to be further elucidated.
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