Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 426904
Title Arabinoxylans and inulin differentially modulate the mucosal and luminal gut microbiota and mucin-degradation in humanized rats
Author(s) Abbeele, P. van den; Gerard, P.; Rabot, S.; Bruneau, A.; Aidy, S. El; Derrien, M.M.N.; Kleerebezem, M.; Zoetendal, E.G.; Smidt, H.; Verstraete, W.; Wiele, T. van der; Possemiers, S.
Source Environmental Microbiology 13 (2011)10. - ISSN 1462-2912 - p. 2667 - 2680.
DOI https://doi.org/10.1111/j.1462-2920.2011.02533.x
Department(s) Microbiological Laboratory
Host Microbe Interactomics
VLAG
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) chain fatty-acids - 16s ribosomal-rna - gradient gel-electrophoresis - butyrate-producing bacterium - human gastrointestinal-tract - human intestinal microbiota - inflammatory-bowel-disease - in-vitro fermentation - fecal microbiota - human feces
Abstract The endogenous gut microbiota affects the host in many ways. Prebiotics should favour beneficial intestinal microbes and thus improve host health. In this study, we investigated how a novel class of potential prebiotic long-chain arabinoxylans (LC-AX) and the well-established prebiotic inulin (IN) modulate the gut microbiota of humanized rats. Six weeks after axenic rats were inoculated with a human faecal microbiota, their colonic microbiota was similar to this inoculum (similar to 70%), whereas their caecal microbiota was enriched with Verrucomicrobia and Firmicutes concomitant with lower abundance of Bacteroidetes. Moreover, different Bifidobacterium species colonized the lumen (B. adolescentis) and mucus (B. longum and B. bifidum). Both LC-AX and IN increased SCFA levels and induced a shift from acetate towards health-promoting propionate and butyrate respectively. By applying a high-resolution phylogenetic micro-array (HITChip) at the site of fermentation (caecum), IN and LC-AX were shown to stimulate bacterial groups with known butyrate-producers (Roseburia intestinalis, Eubacterium rectale, Anaerostipes caccae) and bifidobacteria (B. longum) respectively. Prebiotic administration also resulted in lower caecal abundances of the mucin-degrading Akkermansia muciniphila and potentially more mucin production by the host. Both factors might explain the increased caecal mucin levels for LC-AX (threefold) and IN (sixfold). These mucins were degraded along the colon, resulting in high faecal abundances of Akkermansia muciniphila for LC-AX and especially IN-treated rats. Finally, the microbial changes caused an adaptation period for the host with less weight gain, after which the host fine-tuned the interaction with this altered microbiota. Our results demonstrate that next to IN, LC-AX are promising prebiotic compounds by stimulating production of health-promoting metabolites by specific microbes in the proximal regions. Further, prebiotic supplementation shifted mucin degradation to distal regions, where mucin-degraders may produce beneficial metabolites (e. g. propionate by Akkermansia muciniphila), so that prebiotics may potentially improve gut health along the entire length of the intestine
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