Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 426927
Title Enzyme assisted protein extraction from rapeseed, soybean, and microalgae meals
Author(s) Sari, Y.W.; Bruins, M.E.; Sanders, J.P.M.
Source Industrial Crops and Products 43 (2013). - ISSN 0926-6690 - p. 78 - 83.
DOI http://dx.doi.org/10.1016/j.indcrop.2012.07.014
Department(s) Biobased Chemistry and Technology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) functional-properties - soy protein - antioxidant capacity - conversion factors - phenolic-acids - products - chemicals - biomass - alkaline - heat
Abstract Oilseed meals that are by-products from oil production are potential resources for protein. The aim of this work is to investigate the use of enzymes in assisting in the extraction of protein from different oilseed meals, namely rapeseed, soybean, and microalgae meals. In addition, microalgae without prior oil removal was also tested. The extraction was performed varying temperature, pH, and type of enzyme. More protein was extracted at alkaline conditions, compared to acidic conditions. At alkaline pH, 80% protein of soybean meal and 15–30% protein of rapeseed and microalgae meals was extracted without enzyme addition. The addition of enzyme under this condition increased protein extraction yield to 90% for soybean meal and 50–80% for rapeseed and microalgae meals. Here, Protex 40XL, Protex P, and Protex 5L that work at alkaline pH assisted protein extraction particularly for rapeseed and microalgae meals. Microalgae without prior oil removal had the lowest protein extraction yield, illustrating that oil removal prior to protein extraction is beneficial for protein recovery. In general, protein extraction was influenced by pH, the type of biomass, and the addition of enzyme, but not by the type of enzyme that was used.
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