Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 427183
Title Selecting optimal conditions for Alcalase CLEA-OM for synthesis of dipeptides in organic media
Author(s) Vossenberg, P.; Beeftink, H.H.; Nuijens, T.; Cohen Stuart, M.A.; Tramper, J.
Source Journal of Molecular Catalysis. B, Enzymatic 75 (2012)3. - ISSN 1381-1177 - p. 43 - 49.
DOI https://doi.org/10.1016/j.molcatb.2011.11.008
Department(s) Bioprocess Engineering
Physical Chemistry and Colloid Science
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) industrial protease alcalase - controlled peptide-synthesis - controlled water activity - enzymatic-synthesis - support material - solvents - transesterification - acetonitrile - tripeptide - catalysis
Abstract In protease-catalyzed peptide synthesis, the availability of water is essential, as a compromise must be made between on the one hand the overall enzymatic activity and, on the other hand, the rate of product synthesis. Water is essential for enzyme activity, but at the same time causes hydrolytic side reactions. We studied the coupling of the carbamoylmethyl ester of N-protected phenylalanine and phenylalanine amide in tetrahydrofuran catalyzed by Alcalase CLEA-OM at a range of water activity (aw) values, including the coupling in the presence of molecular sieves (i.e. at very low aw values). The hydrolytic side reaction (in the present system only the hydrolysis of substrate occurs) was found to dominate above an aw value of about 0.2. To prevent hydrolysis, the presence of molecular sieves was found to be necessary.
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