Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 427237
Title Anaerobic benzene degradation under denitrifying conditions: Peptococcaceae was identified as dominant benzene degrader by Stable Isotope Probing (SIP)
Author(s) Zaan, B.M. van der; Talarico Saia, F.; Plugge, C.M.; Vos, W.M. de; Smidt, H.; Stams, A.J.M.; Langenhoff, A.A.M.; Gerritse, J.
Source Environmental Microbiology 14 (2012)5. - ISSN 1462-2912 - p. 1171 - 1181.
DOI https://doi.org/10.1111/j.1462-2920.2012.02697.x
Department(s) Microbiological Laboratory
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) reducing enrichment culture - molecular characterization - microbial communities - contaminated aquifer - nitrate reduction - sequence data - bacteria - identification - oxidation - biodegradation
Abstract An anaerobic microbial community was enriched in a chemostat that was operated for more than 8 years with benzene and nitrate as electron acceptor. The coexistence of multiple species in the chemostat and the presence of a biofilm, led to the hypothesis that benzene-degrading species coexist in a syntrophic interaction, and that benzene can be degraded in syntrophy by consortia with various electron acceptors in the same culture. The benzene-degrading microorganisms were identified by DNA-stable isotope probing with [U-(13) C]-labelled benzene, and the effect of different electron donors and acceptors on benzene degradation was investigated. The degradation rate constant of benzene with nitrate (0.7 day(-1) ) was higher than reported previously. In the absence of nitrate, the microbial community was able to use sulfate, chlorate or ferric iron as electron acceptor. Bacteria belonging to the Peptococcaceae were identified as dominant benzene consumers, but also those related to Rhodocyclaceae and Burkholderiaceae were found to be associated with the anaerobic benzene degradation process. The benzene degradation activity in the chemostat was associated with microbial growth in biofilms. This, together with the inhibiting effect of hydrogen and the ability to degrade benzene with different electron acceptors, suggests that benzene was degraded via a syntrophic process
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