Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 427289
Title Metagenome analysis reveals yet unexplored reductive dechlorinating potential of Dehalobacter sp. E1 growing in coculture with Sedimentibacter sp
Author(s) Maphosa, F.; Passel, M.W.J. van; Vos, W.M. de; Smidt, H.
Source Environmental Microbiology Reports 4 (2012)6. - ISSN 1758-2229 - p. 604 - 616.
Department(s) Microbiological Laboratory
Systems and Synthetic Biology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) desulfitobacterium-frappieri pcp-1 - complete genome sequence - dehalococcoides spp. - online tool - hafniense - tetrachloroethene - dehalogenation - vitamin-b-12 - restrictus - bacterium
Abstract The importance of Dehalobacter species in bioremediation as dedicated degraders of chlorinated organics has been well recognized. However, still little is known about Dehalobacter's full genomic repertoires, including the genes involved in dehalogenation. Here we report the first insights into the genome sequence of Dehalobacter sp. E1 that grows in strict co-culture with Sedimentibacter sp. B4. Based on the co-culture metagenome and the genome of strain B4 (4.2¿Mbp) we estimate the genome sequence of strain E1 to be 2.6¿Mbp. Ten putative reductive dehalogenase homologue (Rdh)-encoding gene clusters were identified. One cluster has a putative tetrachloroethene Rdh-encoding gene cluster, similar to the pceABCT operon previously identified in Dehalobacter restrictus. Metagenome analysis indicated that the inability of strain E1 to synthesize cobalamin, an essential cofactor of reductive dehalogenases, is complemented by Sedimentibacter. The metagenomic exploration described here maps the extensive dechlorinating potential of Dehalobacter, and paves way for elucidation of the interactions with its co-cultured Sedimentibacter
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