Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 427833
Title Evaluation of Design Strategies for Time Course Experiments in Genetic Networks: Case Study of the XlnR Regulon in Aspergillus niger
Author(s) Omony, J.; Mach-Aigner, A.R.; Graaff, L.H. de; Straten, G. van; Boxtel, A.J.B. van
Source IEEE/ACM Transactions on Computational Biology and Bioinformatics 9 (2012)5. - ISSN 1545-5963 - p. 1316 - 1325.
DOI https://doi.org/10.1109/TCBB.2012.59
Department(s) Systems and Control Group
Systems and Synthetic Biology
Biomass Refinery and Process Dynamics
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) parametric sensitivity control - systems biology - signaling pathway - identifiability - identification - components - reveals - models
Abstract One of the challenges in genetic network reconstruction is finding experimental designs that maximize the information content in a data set. In this paper, the information value of mRNA transcription time course experiments was used to compare experimental designs. The study concerns the dynamic response of genes in the XlnR regulon of Aspergillus niger, with the goal to find the best moment in time to administer an extra pulse of inducing D-xylose. Low and high D-xylose pulses were used to perturb the XlnR regulon. Evaluation of the experimental methods was based on simulation of the regulon. Models that govern the regulation of the target genes in this regulon were used for the simulations. Parameter sensitivity analysis, the Fisher Information Matrix (FIM) and the modified E-criterion were used to assess the design performances. The results show that the best time to give a second D-xylose pulse is when the D-xylose concentration from the first pulse has not yet completely faded away. Due to the presence of a repression effect the strength of the second pulse must be optimized, rather than maximized. The results suggest that the modified E-criterion is a better metric than the sum of integrals of absolute sensitivity for comparing alternative designs
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