Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 427970
Title Self-packed filter plates: a good alternative for pre-packed filter plates for developing purification processes of therapeutic proteins
Author(s) Li, X.; Roo, G. de; Burgers, K.; Ottens, M.; Eppink, M.H.M.
Source Biotechnology Journal 7 (2012)10. - ISSN 1860-6768 - p. 1269 - 1276.
DOI http://dx.doi.org/10.1002/biot.201200045
Department(s) Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) chromatographic-separations - throughput - design
Abstract The use of high throughput screening (HTS) has successfully been applied in the past years in downstream process development of therapeutic proteins. Different HTS applications were introduced to speed up the purification process development of these proteins. In the light of these findings, studies were conducted to develop a controlled way of pipetting the right amount of resin in self-packed filter plates. In total thirteen plates were tested for suitability in a HTS application, based upon the different studies the Seahorse plate was experimentally verified as the best filter plate. Suitable conditions to prepare a self-packed plate were established so that linear correlations between resin concentration (POROS 50HS) and UV adsorption (420 nm) were obtained and the accuracy of slurry dispensing in a micro-titer plate was controlled within 1% deviation under established conditions. Overall, self packed filter plates are equipped to be used in the development of purification processes in HTS mode.
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