Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428085
Title Proteomic analysis of latex from the rubber-producing plant Taraxacum brevicorniculatum
Author(s) Wahler, D.; Colby, T.; Kowalski, N.A.; Harzen, A.; Wotzka, S.Y.; Hillebrand, A.; Fischer, R.; Helsper, J.P.F.G.; Schmidt, J.; Schulze Gronover, C.; Prüfer, D.
Source Proteomics 12 (2012)6. - ISSN 1615-9853 - p. 901 - 905.
Department(s) PRI BIOS Applied Genomics & Proteomics
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) laticifers - extraction
Abstract Many plants produce latex, a specialized, metabolically active cytoplasm. This is generally regarded as a defensive trait but latex may also possess additional functions. We investigated the role of latex in the dandelion species Taraxacum brevicorniculatum that contains considerable amounts of high-quality natural rubber by carrying out a comprehensive analysis of the latex proteome. We developed reliable protocols for the preparation of protein samples for one-dimensional gel electrophoresis, two-dimensional gel electrophoresis, and subsequent mass spectrometry analysis, which led to 278 unique identifications. A gene ontology classification system based on comparisons with known Arabidopsis thaliana root proteins showed that dandelion proteins involved in lipid metabolism and transport were enriched in the latex proteome, whereas those involved in stress responses were not. We also found that proteins involved in rubber biosynthesis were distributed among different fractions of the latex proteome
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