Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428110
Title 2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves
Author(s) Falvo, S.; Carli, M. Di; Desiderio, A.; Benvenuto, E.; Moglia, A.; America, A.H.P.; Lanteri, S.; Acquadro, A.
Source Proteomics 12 (2012)3. - ISSN 1615-9853 - p. 448 - 460.
DOI https://doi.org/10.1002/pmic.201100337
Department(s) PRI BIOS Applied Genomics & Proteomics
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) ultraviolet-b radiation - heat-shock proteins - proteome analysis - plants - stress - metabolism - rice - photorespiration - photooxidation - biosynthesis
Abstract Plants respond to ultraviolet stress inducing a self-defence through the regulation of specific gene family members. The UV acclimation is the result of biochemical and physiological processes, such as enhancement of the antioxidant enzymatic system and accumulation of UV-absorbing phenolic compounds (e.g. flavonoids). Globe artichoke is an attractive species for studying the protein network involved in UV stress response, being characterized by remarkable levels of inducible antioxidants. Proteomic tools can assist the evaluation of the expression patterns of UV-responsive proteins and we applied the difference in-gel electrophoresis (DIGE) technology for monitoring the globe artichoke proteome variation at four time points following an acute UV-C exposure. A total of 145 UV-C-modulated proteins were observed and 119 were identified by LC-MS/MS using a ~144¿000 customized Compositae protein database, which included about 19¿000 globe artichoke unigenes. Proteins were Gene Ontology (GO) categorized, visualized on their pathways and their behaviour was discussed. A predicted protein interaction network was produced and highly connected hub-like proteins were highlighted. Most of the proteins differentially modulated were chloroplast located, involved in photosynthesis, sugar metabolisms, protein folding and abiotic stress. The identification of UV-C-responsive proteins may contribute to shed light on the molecular mechanisms underlying plant responses to UV stress
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