Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 428132
Title Endoplasmic reticulum-quality control chaperones facilitate the biogenesis of cf receptor-like proteins involved in pathogen resistance of tomato
Author(s) Liebrand, T.W.H.; Smit, P.; Abd-El-Haliem, A.; Jonge, R. de; Cordewener, J.H.G.; America, A.H.P.; Sklenar, J.; Jones, A.M.; Robatzek, S.; Thomma, B.P.H.J.; Tameling, W.I.; Joosten, M.H.A.J.
Source Plant Physiology 159 (2012)4. - ISSN 0032-0889 - p. 1819 - 1833.
DOI http://dx.doi.org/10.1104/pp.112.196741
Department(s) Laboratory of Phytopathology
PRI BIOS Applied Genomics & Proteomics
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) defective brassinosteroid receptor - pattern-recognition receptors - plant innate immunity - cladosporium-fulvum - disease resistance - hypersensitive response - virulence factor - effector ecp6 - cell-death - gene
Abstract Cf proteins are receptor-like proteins (RLPs) that mediate resistance of tomato (Solanum lycopersicum) to the foliar pathogen Cladosporium fulvum. These transmembrane immune receptors, which carry extracellular leucine-rich repeats that are subjected to posttranslational glycosylation, perceive effectors of the pathogen and trigger a defense response that results in plant resistance. To identify proteins required for the functionality of these RLPs, we performed immunopurification of a functional Cf-4-enhanced green fluorescent protein fusion protein transiently expressed in Nicotiana benthamiana, followed by mass spectrometry. The endoplasmic reticulum (ER) heat shock protein70 binding proteins (BiPs) and lectin-type calreticulins (CRTs), which are chaperones involved in ER-quality control, were copurifying with Cf-4-enhanced green fluorescent protein. The tomato and N. benthamiana genomes encode four BiP homologs and silencing experiments revealed that these BiPs are important for overall plant viability. For the three tomato CRTs, virus-induced gene silencing targeting the plant-specific CRT3a gene resulted in a significantly compromised Cf-4-mediated defense response and loss of full resistance to C. fulvum. We show that upon knockdown of CRT3a the Cf-4 protein accumulated, but the pool of Cf-4 protein carrying complex-type N-linked glycans was largely reduced. Together, our study on proteins required for Cf function reveals an important role for the CRT ER chaperone CRT3a in the biogenesis and functionality of this type of RLP involved in plant defense
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