Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428457
Title Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars
Author(s) Wang, Yue; Kronenburg-van de Ven, B.C.E. van; Menzel, T.R.; Maliepaard, C.A.; Shen, X.; Krens, F.A.
Source Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants 111 (2012)1. - ISSN 0167-6857 - p. 113 - 122.
Department(s) WUR Plant Breeding
Laboratory of Entomology
Laboratory of Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) triticum-aestivum l - lilium-longiflorum - plant-regeneration - beta-glucuronidase - transgenic wheat - inhibitor(s) - floriculture - expression - cultures - gene
Abstract To pursue genetic improvement of lily, efficiency of both regeneration and transformation from callus cultures induced from different explants were evaluated in multiple cultivars. Thirty-five callus lines induced from filaments or styles and one control callus line derived from bulb scales of in total twenty lily cultivars representing Lilium longiflorum, Oriental × Trumpet and Longiflorum × Asiatic hybrids were maintained on a medium with 8.3 µM picloram (PIC). In this study, they were tested for their regeneration potential by transferring them onto a regeneration medium supplemented with 0.4 µM PIC and 0.044 µM 6-benzyladenine. Regeneration was obtained in all cultivars examined and the percentage varied from zero to 89 % in the 36 callus lines. Regeneration frequency was significantly influenced by the genotype (cultivar). Subculturing the calli every 4 weeks by refreshing the regeneration medium contributed positively to bulblet formation, when compared to an eight week subculture frequency. It was found that the regeneration ability generally decreased with an increasing age of the callus cultures for all cultivars. The origin of the callus (style or filament) did not lead to significant differences in regeneration frequency, but there was an interaction between callus origin and genotype. Calli of eight randomly chosen cultivars were co-cultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors with the gus gene as reporter and putative transgenic plants were produced. GUS histochemical assays demonstrated transient and stable expression of the gus gene in both calli and regenerated lily plants. Transient expression frequencies ranged from 0.3 to 20.6 % while stable transformation was much lower, only 1.4 % as the maximum
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