Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428565
Title Extended-spectrum-ß-lactamase- and AmpC-ß-lactamase-producing Escherichia coli in Dutch broilers and broiler farms
Author(s) Dierikx, C.M.; Goot, J.A. van der; Fabri, T.; Essen-Zandbergen, A. van; Smith, H.E.; Mevius, D.J.
Source Journal of Antimicrobial Chemotherapy 68 (2013)1. - ISSN 0305-7453 - p. 60 - 67.
Department(s) CVI Bacteriology and Epidemiology
CVI Diagnostics and Crisis
CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) salmonella-enterica - resistance genes - plasmids - humans - food - animals - poultry - chicken - enterobacteriaceae - identification
Abstract Objectives The aim of this study was to establish the prevalence of extended-spectrum ß-lactamase (ESBL)- and AmpC ß-lactamase-producing Escherichia coli at Dutch broiler farms and in farmers and to compare ESBL/AmpC-producing isolates from farmers and their animals. Methods Twenty-five to 41 cloacal swabs collected from broilers at each of 26 farms and 18 faecal samples from 18 broiler farmers were analysed for determination of the presence of ESBL/AmpC-producing E. coli. ESBL/AmpC genes were characterized by microarray, PCR and sequencing. Plasmids were characterized by transformation and PCR-based replicon typing. Subtyping of plasmids was done by plasmid multilocus sequence typing or restriction fragment length polymorphism. E. coli genotypes were determined by multilocus sequence typing. Results Birds from all farms were positive for ESBL/AmpC-producing E. coli, and on 22/26 farms the within-farm prevalence was =80%. Six of 18 farmers carried isolates containing ESBL/AmpC genes blaCTX-M-1, blaCMY-2 and/or blaSHV-12, which were also present in the samples from their animals. In five of these isolates, the genes were located on identical plasmid families [IncI1 (n¿=¿3), IncK (n¿=¿1) or IncN (n¿=¿1)], and in isolates from two farmers the genes were carried on identical plasmid subtypes (IncI1 ST12 and IncN ST1, where ST stands for sequence type) as in the isolates from their animals. Conclusions This study shows a high prevalence of birds carrying ESBL/AmpC-producing E. coli at Dutch broiler farms and a high prevalence of ESBL/AmpC-producing E. coli in farmers. This is undesirable due to the risk this poses to human health. Future research should focus on identification of the source of these isolates in the broiler production chain to make interventions resulting in reduction of these isolates possible.
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