Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428715
Title Cleavage of group 1 coronavirus spike proteins: how furin cleavage is traded off against heparan sulfate binding upon cell culture adaptation
Author(s) Haan, C.A.M. de; Haijema, B.J.; Schellen, P.; Wichgers Schreur, P.J.; Lintelo, te, E.; Vennema, H.; Rottier, P.J.M.
Source Journal of Virology 82 (2008)12. - ISSN 0022-538X - p. 6078 - 6083.
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) feline infectious peritonitis - extended host-range - bacteriophage-t7 rna-polymerase - murine coronavirus - proteolytic cleavage - functional-characterization - enteric coronavirus - endoprotease furin - sars coronavirus - aminopeptidase-n
Abstract A longstanding enigmatic feature of the group 1 coronaviruses is the uncleaved phenotype of their spike protein, an exceptional property among class I fusion proteins. Here, however, we show that some group 1 coronavirus spike proteins carry a furin enzyme recognition motif and can actually be cleaved, as demonstrated for a feline coronavirus. Interestingly, this feature can be lost during cell culture adaptation by a single mutation in the cleavage motif; this, however, preserves a heparan sulfate binding motif and renders infection by the virus heparan sulfate dependent. We identified a similar cell culture adaptation for the human coronavirus OC43
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