Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428893
Title EU-Approved Rapid Tests for Bovine Spongform Encephalopathy Detect Atypical Forms: A Study for Their Sensitivities
Author(s) Meloni, D.; Davidse, A.; Langeveld, J.P.M.; varello, K.; Casalone, C.; Corona, C.; Balkema-Buschmann, A.; Groschup, M.; Ingravalle, F.; Bozzetta, E.
Source PLoS One 7 (2012)9. - ISSN 1932-6203 - 12 p.
DOI http://dx.doi.org/10.1371/journal.pone.0043133
Department(s) CVI Bacteriology and Epidemiology
CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) creutzfeldt-jakob-disease - bse-affected cattle - prion protein - scrapie - transmission - switzerland - primate - strain - prp
Abstract Since 2004 it become clear that atypical bovine spongiform encephalopthies (BSEs) exist in cattle. Whenever their detection has relied on active surveillance plans implemented in Europe since 2001 by rapid tests, the overall and inter-laboratory performance of these diagnostic systems in the detection of the atypical strains has not been studied thoroughly to date. To fill this gap, the present study reports on the analytical sensitivity of the EU-approved rapid tests for atypical L-and H-type and classical BSE in parallel. Each test was challenged with two dilution series, one created from a positive pool of the three BSE forms according to the EURL standard method of homogenate preparation (50% w/v) and the other as per the test kit manufacturer's instructions. Multilevel logistic models and simple logistic models with the rapid test as the only covariate were fitted for each BSE form analyzed as directed by the test manufacturer's dilution protocol. The same schemes, but excluding the BSE type, were then applied to compare test performance under the manufacturer's versus the water protocol. The IDEXX HerdChek (R) BSE-scrapie short protocol test showed the highest sensitivity for all BSE forms. The IDEXX (R) HerdChek BSE-scrapie ultra short protocol, the Prionics (R) - Check WESTERN and the AJ Roboscreen (R) BetaPrion tests showed similar sensitivities, followed by the Roche (R) PrionScreen, the Bio-Rad (R) TeSeE (TM) SAP and the Prionics (R) - Check PrioSTRIP in descending order of analytical sensitivity. Despite these differences, the limit of detection of all seven rapid tests against the different classes of material set within a 2 log(10) range of the best-performing test, thus meeting the European Food Safety Authority requirement for BSE surveillance purposes. These findings indicate that not many atypical cases would have been missed surveillance since 2001 which is important for further epidemiological interpretations of the sporadic character of atypical forms.
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